Thp1 lipofectamine 3000
WebJun 5, 2015 · THP-1 cells with a stable, individual ... 350 μg/mL L-glutamine) and incubated for 5 min at RT. Next, lipofectamine 2000-containing solutions were added to the diluted … WebApr 12, 2024 · Lentivirus was produced via transient transfection of 293 T cells with transfer plasmid, along with LV-MAX lentiviral packaging mix (Gibco, cat. no. A43237) using Lipofectamine 3000 transfection ...
Thp1 lipofectamine 3000
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Websequencing. Each clone was transfected into THP-1 cells in duplicate (500 ng per well) using Lipofectamine 3000 and Opti-MEM. Additionally, phRL-TK Renilla luciferase reporter …
WebApr 11, 2024 · Purpose Reprogrammed lipid metabolism is a hallmark of cancer that provides energy, materials, and signaling molecules for rapid cancer cell growth. Cancer … WebApr 19, 2024 · Overexpression of CMPK2 in THP-1 cells. The human CMPK2-GFP gene was a kind gift from Dr. Chang, Zee-Fen, professor in National Taiwan University. Expression of …
WebTHP1 cells were incubated with MBP-TcpB or MBP alone followed by treatment of cells with PFA. TcpB did not affect the cell death induced by PFA, which suggests that TcpB attenuates cytotoxicity induced by inflammatory caspases ... (3 × 10 6) were transfected with pCMV-FLAG-caspase-4 using Lipofectamine 3000 reagent (Invitrogen) in 60-mm … WebApr 12, 2024 · The expression of NLRP10 was significantly knocked down by three synthesized siRNAs (P < 0.05; Fig. 1C).Reduction of NLRP10 led to significantly reduced inflammatory cytokines (IL‐6, IL‐1β) release under stimulation with AGEs (P < 0.05; Fig. 1D).Additionally, the second siRNA was chosen for the following experiments, since it …
Web2 days ago · Transfection of plasmids was done using Lipofectamine 2000 or Lipofectamine 3000 according to the manufacturer’s instructions. Flow cytometry analyses of transfected cells were typically ...
Web对照组细胞不进行处理，miR-NC组按Lipofectamine 3000说明书方法将miR-NC转染到THP-1细胞，miR-34a inhibitor组按Lipofectamine 3000说明书方法将miR-34a inhibitor转染 … christophe nadineWeb2细胞毒性低图2.LipoFectMax™，LipoFectamine®2000及LipoFectamine®3000分别对A549细胞进行转染操作，通过计算转染后的细胞存活率比较 ... 7月17日收录于话 … getting age from birthdate in excelWebThe current standard-of-care treatment for glioblastoma includes DNA damaging agents, γ-irradiation (IR) and temozolomide (TMZ). These treatments fail frequently and there is limited alternative strategy. Therefore, identifying a new therapeutic getting a ged in montgomery countyWebCell Type. Established Cell Lines, Stem Cells, Primary Cells, Hard-to-Transfect Cells. Content And Storage. Contains one vial of Lipofectamine™ 3000 Reagent (1.5 mL) and one vial of … christophe naessensWeb双荧光素酶验证实验转染问题. 最近在做双荧光素酶验证miRNA是否与靶基因的3UTR结合，目前有个疑问，在进行转染时准备使用lipo2000，在24孔板中进行实验，在转染时需要报告载体和miRNA模拟物一起转进细胞，那么在转染. 丁香园-核酸基因技术-2024-03-10 16:16:22 getting a ged in washington stateWebI am currently trying to tranfect THP1 adherent cells (PMA differentiated for 10 hrs) with specific vendor protocol. But it resulted a big cell death and also poor tranfection yield. I … christophe naegelen remiremontWebApr 11, 2024 · GC cells were treated with the recombinant vector and corresponding empty vector using the Lipofectamine TM 3000 Transfection Kit (Thermo-Fisher, USA). 2.4. ... PMA treated THP-1-derived-IL-6 promotes EMT of SW48 through STAT3/ERK-dependent activation of Wnt/β-catenin-catenin signaling pathway. Biomed Pharmacother, 108 ... getting age in excel