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Thp1-blue isg

WebNov 1, 2014 · THP1-Blue™ ISG cells are derived from THP-1 cell line by stable integration of an interferon regulatory factor (IRF)-inducible SEAP reporter construct. THP1-Blue™ ISG cells secrete SEAP under the control of an ISG54 promoter in conjunction with IFN-stimulated elements resulting in high responsiveness to PRR agonists that trigger the IFN … WebDec 5, 2024 · THP1-Blue ISG cells express functional STING, and, upon stimulation with a STING ligand, such as cyclic di-AMP, activate IRF signaling and IRF-driven expression of a SEAP reporter. The THP1 reporter cells were treated with clarified lysates from 293T cells transfected with mammalian expression plasmids for human codon optimized sequences …

Buy THP1-Blue™ ISG Cells Axil Scientific

WebApr 18, 2024 · THP1-Blue™ ISG Cells : Human THP-1 Monocytes - IRF SEAP Reporter: THP1-Lucia™ ISG Cells : Human THP-1 Monocytes - IRF Luciferase Reporter Cells: THP1-Blue™ … http://www.qfbio.com/shqf-Article-2624279/ the outer worlds sanjar https://gradiam.com

THP1 Blue ISG Cells - Gentaur.com

Webj) STING activation of human monocyte-derived THP1-Blue ISG cells measured after overnight incubation. All data are presented as mean ± SEM, showing representative results from two independent studies with n = 3, with an exception of g) with n = 1. Scale bars in a) and b) = 100 nm, h) = 5 µm. WebProduct information: THP1 Lucia ISG Cells - Gentaur.com - Product info. THP1-Lucia™ ISG Cells. Catalog number. thpl-isg. Price. Please ask . ... THP1-Blue™ ISG Cells. Catalog number: thp-isg. Size: 3-7 x 10e6 cells. Price: Please ask. THP1-Dual™ Cells. Catalog number: thpd-nfis. Size: 3-7 x 10e6 cells. WebDec 12, 2016 · THP1-Blue ISG-KD-STING cells are derivatives of THP1-Blue ISG cells with stable knockdown of the gene encoding STING, which results in ∼ 50% reduced STING … shumi threading spa

Potential Therapeutic Value of the STING Inhibitors

Category:THP1-Dual™ (NF-kB, ISG) Cells - jlresearch.com.au

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Thp1-blue isg

Inhibition of innate immune cytosolic surveillance by an M

WebJul 1, 2024 · THP1 blue ISG cells (3000–5000/well) were seeded into 96-well plates and cultured overnight and then different concentration of compounds in fresh medium were added. The SEAP activity was measured by using the QUANTI-Blue kit (invivogen) after 24 h according to the manufacturer’s instructions. WebTHP1-Blue™ ISG cells THP1-Blue™ ISG cells are derived from THP-1 cell line by stable integration of an interferon regulatory factor (IRF)-inducible SEAP reporter construct. THP1-Blue™ ISG cells secrete SEAP under the control of an ISG54 promoter in conjunction with IFN-stimulated elements Besifloxacin ...

Thp1-blue isg

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WebTHP1-Blue® ISG cells were specifically designed for monitoring the interferon signaling pathway in a physiologically relevant cell line. THP1-Blue® were derived from the human THP-1 monocyte cell line by stable integration of an interferon regulatory factor (IRF)-inducible SEAP reporter construct. Web一类杂环取代的苯并咪唑二聚体或其药学上可接受的盐、组合物及其用途专利检索,一类杂环取代的苯并咪唑二聚体或其药学上可接受的盐、组合物及其用途属于抗肿瘤药专利检索, …

WebTHP1-Blue™ ISG Cells from InvivoGen. Product Specs; Item THP1-Blue™ ISG Cells; Company InvivoGen; Catalog Number thp-isg; This product is no longer available on … WebMar 25, 2024 · THP1-Blue ISG cells (Invivogen) were cultured in RPMI1640 and 10% hi-FBS, 55 μM 2-mercaptoethanol, 50 μg ml −1 gentamicin sulfate and 100 μg ml −1 Zeocin (Invivogen).

WebUS11597746B2 US17/260,886 US202417260886A US11597746B2 US 11597746 B2 US11597746 B2 US 11597746B2 US 202417260886 A US202417260886 A US … WebOct 15, 2024 · THP1 ISG Reporter Activity. Wildtype THP1-Blue™ ISG cells were differentiated in a 96-well plate (5 x 10 4 cells/well) with PMA as described above. Cells …

Webj) STING activation of human monocyte-derived THP1-Blue ISG cells measured after overnight incubation. All data are presented as mean ± SEM, showing representative …

WebTHP1-Blue ISG-KD-STING (Q54972361) From Wikidata. Jump to navigation Jump to search. cell line. edit. Language Label Description Also known as; English: THP1-Blue ISG-KD … shumi tribe ff8 stonesWebSep 4, 2024 · The reporter THP1-blue ISG cells were obtained from InvivoGen and cultured in complete RPMI1640 medium supplemented with 10% FBS, GlutaMAX (Gibco), 100 … shumi village water stoneWebCVCL_X589 (THP1-Blue ISG-KD-STING) CVCL_X585 (THP1-Blue NF-kappaB) CVCL_X599 (THP1-Dual) CVCL_A8BE (THP1-HMGB1-Lucia) CVCL_X587 (THP1-Lucia ISG) CVCL_X590 (THP1-Lucia NF-kappaB) CVCL_5I77 (THP1-NLRC4) CVCL_X601 (THP1-Null) CVCL_A8AK (THP1-Null2) CVCL_X582 (THP1-Xblue) CVCL_A0HA (THP18) Sex of cell: Male: Age at … shumi toys and gifts reviewWebJan 13, 2024 · THP1-Blue ISG cells express functional STING, and, upon stimulation with a STING ligand, such as cyclic di-AMP, activate IRF signaling and IRF-driven expression of a SEAP reporter. The THP1 reporter cells were treated with clarified lysates from 293T cells transfected with mammalian expression plasmids for human codon optimized sequences … the outer worlds save locationWebCharacteristics of THP1-ISG, HEK-Blue TLR7, TLR8, and TLR9. A) ISG-inducing activity of various TLR9 ligands. ISG-inducing activity of various TLR9 ligands used in Figure 2B was … the outer worlds science skillWebNov 10, 2014 · The A2a + human monocyte line THP1-Blue-ISG-hSEAP (InvivoGen, Montaudran, France) was cultured as described above. Upon activation of human STING, … the outer worlds saveWebTHP1-Dual™ cells were derived from the human THP-1 monocyte cell line by stable integration of two ... (Lucia). Both reporter proteins are readily measurable in the cell … the outer worlds save game location