http://www.bioon.com.cn/doc/showarticle.asp?newsid=101857 WebbDescription pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for …

pHis2酵母单杂交载体 631903报价/价格pHis2酵母单杂交载体质粒 …

Webb20 juni 2024 · 资源涵盖克隆载体、真核表达载体、原核表达载体、病毒载体、信号通路报告载体、亚细胞定位载体、荧光蛋白载体、昆虫细胞表达载体、启动子载体、诱导调控载体等十多种类型上万个品种。. 我们拥有目前中国最大的cDNA克隆库、覆盖30,000个人全长基因 … Webb2 Visualize Your Process Cloning is easier when you can see what you are doing. The intuitive interface offers you unparalleled visibility into your work, simplifying often … should newborn sleep in bassinet or crib

PT3705-5 (PR32049) pHIS2

Webb1 juni 2024 · Clone the products of the PCR reactions carried out in step 3 into the pHIS2 T-vector obtained in step 5. The T-A ligation system includes 0.2 μg of T-vector of pHIS2, 0.5 μL of PCR products (Y1 + Y3 and Y2 + Y3), 1 μL of 10× ligation buffer, 3 U of T4 ligase, and PEG6000 at final concentration of 10% (w/v). Webb2.制备感受态并将pHis2-DNA转化进入感受态细胞； 3.SD/-Trp平板检测转化结果。 二、自激活检测 1.制备不同3-AT浓度梯度的SD/-His/-Trp平板，3-AT的浓度一般设定在50-100mM … http://www.coolaber.com/usr/2160/File/F/F4671.pdf should newborns have cereal