WebMar 22, 2024 · This process referred above is highly regulated by the nuclear deacetylase SIRT1 (deacetylation at K49 and K51), which helps LC3 translocated from nuclear to cytoplasm (Huang et al., 2015). In HepG2 cells, enhanced binding of SIRT1-LC3 reduces the endogenous LC3 acetylation and SIRT1 inactivation inhibits autophagy (Li X. et al., … Web目的 探讨mTOR抑制剂雷帕霉素对体外培养的瘢痕疙瘩成纤维细胞生物学特性的影响和自噬的作用,以及其对mTOR信号通路和自噬相关非编码RNA表达的调控.方法 以瘢痕疙瘩成纤维细胞为实验模型,经浓度为10、50、100 nmol/L的雷帕霉素处理后,采用MTS法检测细胞增殖;应用流式细胞仪检测细胞凋亡;透射电镜观察 ...
Identifying an essential role of nuclear LC3 for autophagy
WebJul 14, 2024 · Deacetylation of p53 was induced by the mutation of lysine (K) at 382 to arginine (R). (B) Representative western blot and densitometric analyses of p53 protein expression. n = 3. ** p < 0.01 vs. Control group. (C) Representative western blot of p62, LC3-I, LC3-II, acetylated p53 (Ac-p53), and p53. GAPDH was used as an internal … WebMay 31, 2024 · Accordingly, EP300/P300 sequesters LC3 in the nucleus under nutrient-rich conditions, while deacetylation of LC3, catalyzed by the deacetylase SIRT1, results in nucleo-cytoplasmic translocation of ... greenfield chocolate milk
Schematic Model for Deacetylation and Translocation of …
Webthe deacetylation is also required for LC3 to bind with ATG7 for its subsequent lipidation. Therefore, the results impli-cate the nuclear pool of LC3 as the pri-mary source of membrane-conjugated LC3, and a regulation of deacetylation and nucleocytoplasmic translocation of LC3 in priming starved cells for autoph-agy induction. WebThe degree of deacetylation (DD) of chitin/chitosan samples was successfully determined using 1 H NMR spectroscopy. Chitin/chitosan samples were dissolved in a mixture of 25% DCl in D 2 O and chitosan samples in a mixture of 0.25% DCl in D 2 O. The concentration of chitosan in the solution was about 0.5% (w/v). WebJan 11, 2013 · Sustained activation of X-box-binding protein 1 (XBP1) results in endothelial cell (EC) apoptosis and atherosclerosis development. The present study provides evidence that XBP1 mRNA splicing triggered an autophagic response in ECs by inducing autophagic vesicle formation and markers of autophagy BECLIN-1 and microtubule-associated … flume water system