2024 Cisplatin a549 xenograft

Cisplatin a549 xenograft

Author: vgmd

August undefined, 2024

Webas subcutaneous xenograft model in mice. determination, provision of tumor tissue for target validation. Tumor volume, mean values +/- SEM. n=11. Figure 1: Growth of A549 cells Quality Assurance • Routine authentication of tumor cell lines by STR profiling • Mycoplasma testing of implanted tumor cells by PCR just prior to implantation WebMar 17, 2024 · Human NSCLC cell lines (A549, H1299, H1650, SK-MES-1, and Calu-3) and a normal human bronchial epithelial cell line (HBE) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). Cells were cultured in RPMI-1640 medium at 37 °C in a humidified atmosphere of 5 % CO 2.

The 50 % inhibitory concentration (IC50) of cisplatin in A549 …

WebJan 15, 2024 · The effect of DDP or Cor alone or combined between DDP and Cor compared with control on A549 or A549DDP cells xenograft tumor growth. (A) The timeline of A549 or A549DDP cells incubation subsequently treated with drugs. (B) Time course of mice body weight. Time course of A549 (C) or A549DDP (D) cells tumor growth. WebThe A549 cells were suspended in serum-free RPMI 1640 media and inoculated subcutaneously at a density of 5 × 10 6 cells per 150 µL into the right flank region of each mouse. ... Nephrotoxicity of Polycisplatin and Cisplatin. As shown in the above xenograft trials, there was a severe reduction in body weight of the mice treated with cisplatin ... 2溴吡啶密度

Cisplatin-resistant A549 non-small cell lung cancer cells …

WebJan 12, 2024 · Here, we show that this combination enhances the anti-cancer effect of cisplatin in both in vitro cell lines and in vivo xenograft mouse models of cisplatin-sensitive and -resistant lung, ovarian ... WebDec 1, 2024 · Xenograft Nephrotoxicity Antitumor effect 1. Introduction Cisplatin (CDDP) is a highly potent anticancer agent widely used for treating several types of cancers, … WebThe human NSCLC cell line A549 and cisplatin (DDP)-resistant cell line A549/DDP were purchased from Tianjin Saier Biotechnology Co., China, and cultured in RPMI-1640 … 2溴丙烷水解

Enhanced antitumor effect of cisplatin in human NSCLC cells by …

WebDownload Table The 50 % inhibitory concentration (IC50) of cisplatin in A549 and A549/DDP cells 48 h after drug treatment from publication: RNA interferences targeting the Fanconi anemia/BRCA ... WebOct 1, 2024 · A549 human lung carcinoma cells were obtained from the Korean Cell Line Bank (Seoul, Republic of Korea) and cultured in RPMI 1640 medium with 10% … 2溴异丁酰溴三乙胺WebNov 29, 2024 · Background: Cisplatin plus pemetrexed combination therapy is considered the standard treatment for patients with advanced, non-squamous, non-small-cell lung cancer (NSCLC). However, advanced NSCLC has a 5-year survival rate of below 10%, which is mainly due to therapy resistance. 2溴甲苯

"WebFeb 25, 2015 · (A) A549 DDP cells transfected with sCLU shRNA or scramble shRNA were injected subcutaneously into the right flank of nude mice. When tumors reached −100 mm 3 in volume, the mice received daily 200 μl i.p. injections of DDP (4 mg/kg body/wt.,i.p). DDP was administered i.v. once every 3 days. " - Cisplatin a549 xenograft

Cisplatin a549 xenograft

The antitumour effect of CDDP and GA on A549 human xenograft …

WebSep 2, 2024 · In the beginning, under both in vitro and in vivo conditions (A549 and H460 cells as well as A549 xenograft model) treated with external heat before and after intravascular administration of heat-activated thermosensitive liposome containing cisplatin (HTLC), exhibited rapid accumulation of drug in the tumor as well as a subsequent … WebObjective: The purpose of the study was to investigate the role of peroxidase V (Prx V) in Cisplatin-induced apoptosis of A549 cells and its underlying mechanism. Materials and …

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WebMay 18, 2024 · Our study showed that NO.0449-0145 treatment alone was able to overcome cisplatin resistance in A549-DDP cell and sensitize A549-DDP cells to cisplatin treatment, implying that the inhibition of ... WebThe overexpression and knockdown of PUM2 promoted and inhibited tumor growth of xenograft mice, respectively. ... Cell viability of A549 and HCC827 cells treated with exosomes (25 μg/mL) derived from cisplatin-treated A549-R cells or CAFs. Control exosomes (Exo-control) derived from A549 or HCC827 cells’ own culture mediums. ...

WebMay 28, 2024 · In xenograft models, osimertinib significantly inhibited tumor growth, however, as expected, in 50% of mice drug-resistance developed. A combination of osimertinib with pemetrexed or cisplatin prevented or at … WebMay 11, 2012 · We then evaluated the effects of LKB1 on enhancing low-dose cisplatin-mediated antitumor activities in both NSCLC cell lines in vitro and two human A549 lung …

WebCisplatin Relocalizes RNA Binding Protein HuR and Enhances the Oncolytic Activity of E4orf6 Deleted Adenovirus ... To evaluate the cell lysis activity of dl355, cancer (HeLa and A549) and normal (BJ) cells were infected with dl355 (MOI 1,10, 100; vp/cell), and the cell viability was observed at different time intervals (days 2, 4, and 6 ... WebTargeting of the nano‐zinc carrier to A549/DDP or A549 cells in vivo was detected after subcutaneous injection in a mouse xenograft tumor model. A549/DDP or A549 cells (1 × 10 6 cells) in 50 μL of PBS were injected into the armpits of 4‐week‐old nude mice (BALB/c, nu/nu; SIPPR‐BK Laboratory Animal Co. Ltd, Shanghai, China) using a 25 ...

WebCisplatin: CDDP: A549, H358, H460 and Calu-1 cells: ... H460, H661, A549 cells and xenograft mouse models: Upregulating TF and TFR, downregulating SLC7A11 in a p53-dependent manner and increasing intracellular Fe 2+, …

2準位系とはWebJul 15, 2024 · The cisplatin-resistant A549 cell line (A549/DDP), the parent cell line A549, and 293 cells (JRDUN Biotech. Shanghai, China) were maintained in a humidified atmosphere with 5% CO 2 at 37 °C. 2溴吡啶WebApr 8, 2014 · The MTT assay demonstrated that cisplatin exhibits a dose‑dependent cytotoxicity in A549 cells and synergizes with radiation to promote the cell‑killing effect of radiation. In the xenograft mouse model of Lewis cells, cisplatin plus ionizing radiation (IR) (five doses of 2 Gy) yielded the most significant tumor suppression. 2溴苯乙酮WebFurthermore, combined treatment with intravenous injections of Lps-pVAX-LKB1 and intraperitoneal injection of low-dose cisplatin into mice bearing experimental A549 lung metastasis demonstrated significant (P<0.01) decrease in … 2溴丙酸Webas subcutaneous xenograft model in mice. determination, provision of tumor tissue for target validation. Tumor volume, mean values +/- SEM. n=11. Figure 1: Growth of A549 … 2溴丙烷合成1溴丙烷WebJul 15, 2024 · A549/DDP cells with TIMR59 knockdown was more sensitive to cisplatin treatment in a xenograft model. Moreover, A549/DDP cells exhibited increased glucose … 2滴英語WebNov 29, 2024 · This study revealed that cisplatin resistant A549 lung cancer cells can be identified by their increased levels of mitochondrial mass. However, Mito-High cells … 2滑車