Bzr1 chip-seq
WebAug 4, 2024 · Sources for RNA-seq data related to BZR1 are in legend Table 2 ... PIF4, BZR1, HY5, and MYC2 from ChIP-seq experiments . as available at PlantPan3.0 sho w 34 TFs with binding to the . WebThroughput Sequencing (ChIP-seq) and RNA-Sequencing Trevor Nolan, Sanzhen Liu, Hongqing Guo, Lei Li, Patrick Schnable, and Yanhai Yin ... Fig. 1 Comparison of BR-regulated genes from RNA-seq and BES1 and BZR1 target genes identified by ChIP [7, 8, 11]. BR Target Gene Identification by ChIP-seq and RNA-seq. 66
Bzr1 chip-seq
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WebAug 13, 2024 · We generated a first-generation (T 0) transgenic CRISPR-bzr1 line. PCR genotyping and sequencing identified a deletion of 42 bp in proximity to single guide RNA (sgRNA) target sequences ... (E, F) ChIP-qPCR assay of BZR1 binding to the promoters of FER2 and FER3 genes in control and heat stress conditions. WebMay 24, 2024 · Hello, I Really need some help. Posted about my SAB listing a few weeks ago about not showing up in search only when you entered the exact name. I pretty …
WebNov 27, 2024 · Yeast cells with positive DNA-protein interactions were grown on Leu − plates with 100 ng mL −1 aureobasidin A. C, Direct binding of BZR1 to the promoters of ATG2 and ATG6 was investigated using ChIP-qPCR in BZR1OE plants. Six-week-old BZR1OE plants were treated with water or 500 n m BL, and input chromatin was isolated … WebApr 20, 2015 · Comparison of genes upregulated or downregulated by BR or auxin, identified by RNA-seq in roots (Table S1), to the BZR1 ChIP-chip and ChIP-seq data, and the RootMap spatial gene expression data. QC, quiescent center; MZ, meristem zone; TEZ, transition-elongation zone; LEZ, late elongation zone; DZ, differentiation zone.
WebMay 27, 2014 · ChIP-Seq analysis R (CSAR) software was used to identify binding peaks, with parameters (backg = 10, norm = −1, test = 'Ratio', … WebFeb 27, 2024 · ChIP-sequencing analysis, and yeast one-hybrid and dual-luciferase assays demonstrated that the protein binds to the promoters of AP2/EREBP genes (Zm00001d010676 and Zm00001d032077) and inhibits their transcription. cDNA library screening showed that ZmBES1/BZR1-5 interacts with casein kinase II subunit β4 …
WebJul 22, 2012 · Among the six PRE members, PRE1, PRE5 and PRE6 were identified as PIF4 targets in the ChIP-seq analysis (Supplementary Fig. S6a), and confirmed by ChIP–qPCR to be direct targets of both BZR1 and ...
WebChromatin immunoprecipitation (ChIP) is a powerful method for studying TF-DNA interactions in vivo. To identify all binding sites of a TF in the genome, the DNA obtained in ChIP experiments needs to be analyzed by hybridization to genome-tiling microarrays (ChIP-chip) or by next-generation sequencing (ChIP-seq). offline hearts game downloadWeb56371 Ensembl ENSG00000105325 ENSMUSG00000020245 UniProt Q9UM11 Q9R1K5 RefSeq (mRNA) NM_001136197 NM_001136198 NM_016263 NM_019757 RefSeq … offline hearts game freeWebMar 11, 2005 · Lane S is the G + A chemical sequencing reaction. Footprint regions are marked by vertical lines and corresponding sequence. (D) RBSS for optimal BZR1 binding site. Shown on the top is the alignment of all identified sequences, with the matching sequences marked in red. ... we performed chromatin immunoprecipitation (ChIP) … offline heart rate monitorWebDec 14, 2024 · The published BZR1 ChIP-Seq results show that BZR1 exhibited a binding peak on the promoter of EBF1 . To examine whether EBF1 is a target of BZR1, we performed a ChIP-qPCR using the … offline hearts gameWebApr 7, 2012 · variation, as we did for the BZR1 ChIP-chip experiment (17). 1. ... Although several tools for the analysis of ChIP-seq data have been published recently, there is a growing demand, in particular ... myers chadstone shopping centreWebCRY1a-OE, HY5-OE, and BZR1-OE transgenic plants; HY5-RNAi plants; and bzr1 mutant were generated as described previously (49–55). The CRISPR/Cas9 mutants of hy5 and brc1 (brc1 #5, brc1 #9 lines) in the cv. Condine Red background were generated similar to previous studies (20, 36). The mutation sites are shown in SI Appendix, Figs. S4A and S7A. offline help calculatorWebTo generate comparable data sets, we performed BZR1 ChIP-115 Seq analysis using dark-grown seedlings (Figure 1-source data 1B), as used in the ARF6 and 116 PIF4 ChIP-Seq (Oh et al., 2012). Interestingly, large portions of ARF6 binding targets 117 overlapped with either targets of BZR1 (51%) or PIF4 (71%) or both (42%) (Figure 1C). The myers central